Adherence to HEp-2 Cells and Replication in Macrophages ofSalmonella derbyof Human Origin
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چکیده
منابع مشابه
Penetration and replication of Edwardsiella spp. in HEp-2 cells.
The ability of 22 Edwardsiella strains to penetrate and replicate in cultured epithelial cells was initially evaluated by light microscopy methods and by the recovery of gentamicin-resistant (Gmr) bacteria from the Triton X-100 cell lysates of HEp-2-infected monolayers. Giemsa-stained HEp-2 cells revealed the presence of numerous internalized bacteria 3 h postinfection, often appearing as paral...
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Strains of enteroaggregative Escherichia coli (EAggEC) have been implicated in several studies as important agents of persistent diarrhea among infants in the developing world. We have previously shown that the aggregative adherence (AA) property of EAggEC is associated with the presence of a 60-MDa plasmid which confers AA when introduced into E. coli HB101. Here, we report the cloning of the ...
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Adherence of Aeromonas caviae to HEp-2 and Caco-2 cell monolayers was investigated with 24 clinical isolates. Growth phase, temperature, multiplicity of infection and length of incubation affected adherence. Treatment of the bacteria with trypsin, sodium metaperiodate, mechanical shearing and the addition of cytochalasin B and cycloheximide to the monolayer significantly reduced the adherence c...
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Escherichia coli O157:H7 is found in cattle farms and can live in the intestine of healthy cattle. Mostcases of human illnesses including nonbloody diarrhea, hemorrhagic colitis and hemolytic uremic syndromecan be traced, either directly or indirectly, to cattle. One strategy for reducing the risk of EnterohemorrhagicEscherichia coli (EHEC) infections in human is to reduce the prevalence of inf...
متن کاملInhibition of enteropathogenic Escherichia coli (EPEC) adherence to HEp-2 cells by bovine colostrum and milk.
BACKGROUND enteropathogenic Escherichia coli (EPEC) is the main etiological agent of infantile diarrhea in Brazil and other developing countries. Human milk IgA protects newborn intestinal mucosa by inhibiting bacterial adhesion to epithelial cells and this effect is shown by in vitro assays of EPEC adhesion to HEp-2 cultured cells. Bovine milk, if effective in promoting this protection, could ...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1991
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1991.tb01539.x